Research
RNA binding proteins implicated in the addictions
Funding
1R01DA039168-A1 (PI: Bryant) $3,026,929 04/01/2015-03/31/2020 NIH/NIDA: Bridging Genetic variation with Behavior: Molecular and Functional Mechanisms of Quantitative Trait Gene Regulation of the Stimulant and Addictive Properties of Methamphetamine in Mice Use TALENs and CRISPR/Cas9 to make knockout and knockin mice carrying Hnrnph1 and Rufy1 to identify the quantitative trait gene influencing sensitivity to methamphetamine behaviors […]
Posters
Csnk1e project Lisa Goldberg GPN recruitment poster Olga Lacki Fall 2014 UROP poster Food Addiction project Stacey Kirkpatrick ACNP Poster Rufy1 project Camron Bryant WCPG Poster
Publications
Zhou L, Bryant CD, Loudon AS, Palmer AA, Vitaterna MH, Turek FW (2014). The circadian clock gene Csnk1e regulates REM sleep and NREM sleep architecture in mice. In press, Sleep. Bryant CD, Guido MA, Kole LA, Cheng R. The heritability of oxycodone reward and concomitant phenotypes in a LG/J x SM/J F48 mouse advanced intercross […]
Genetic basis of food “addiction” and eating disorder traits
Stacey Kirkpatrick ACNP 2014 Poster
Genetic basis of the placebo response
Dr. Bryant has a longstanding interest in using mice to study the neurobiological basis of the “placebo effect” (Bryant et al., 2009; Drug and Alcohol Dependence), a phenomenon that has been hypothesized to be mediated by the reward expectation. He plans to develop and apply a forward genetic analysis toward Pavlovian conditioning mouse models across […]
Candidate gene testing of Rufy1 and Hnrnph1 for methamphetamine sensitivity
Dr. Bryant recently generated a panel of 17 interval-specific congenic mice to identify a 0.25 Mb locus on chromosome 11 that regulates the locomotor stimulant response to methamphetamine (MA) (manuscript in preparation). This genomic region contains only two possible quantitative trait genes that could be responsible for the QTL: Rufy1 and Hnrnph1. Rufy1, also known […]
Deciphering the roles of casein kinase 1-epsilon and –delta in the motivational properties of substances of abuse and dopaminergic signaling.
Dr. Bryant recently provided direct genetic and pharmacological evidence that casein kinase 1-epsilon (Csnk1e) inhibits whereas casein kinase 1-delta (Csnk1d) facilitates the locomotor response to psychostimulants and opioids (Bryant et al., 2012; Neuropsychopharmacology). These observations suggest at least two possibilities regarding the molecular and cellular mechanisms of these two highly related isoforms on behavior: 1) […]
K99/R00: “Genetic basis of opioid reward and aversion in mice.”
A current focus is to determine the genetic basis of the rewarding properties of opioids in mice by combining quantitative trait locus (QTL) analysis of conditioned place preference (CPP) and transcriptome analysis via RNA-sequencing in genetic reference populations that yield high resolution QTLs. This multi-pronged approach to gene mapping will accelerate the nomination of candidate […]