Introduction to Optical Microscopy, 2nd Edition

Cambridge University Press

This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.

Table of Contents
  1. Introduction
  2. Monochromatic Wave Propagation
  3. Monochromatic Field Propagation Through a Lens
  4. Intensity Propagation
  5. 3D Imaging
  6. Radiometry
  7. Intensity Fluctuations
  8. Detection Noise
  9. Absorption and Scattering
  10. Widefield Microscopy
  11. Interference Microscopy
  12. Optical Coherence Tomography
  13. Fluorescence
  14. Confocal Microscopy
  15. Structured Illumination Microscopy
  16. Multiphoton Microscopy
  17. Multiharmonic Microscopy
  18. Pump-Probe Microscopy
  19. Superresolution
  20. Imaging in Scattering Media

The problem set can be found here.

The solution set can be obtained here  (for instructors only).

Information about 1st edition can be found here.