Publications

See more publications of Prof. Liu in Google Scholar

    • 2021

      1. Naowarojna, Nathchar, Ronghai Cheng, Juan Lopez, Christina Wong, Lu Qiao, and Pinghua Liu. “Chemical modifications of proteins and their applications in metalloenzyme studies.” Synthetic and systems biotechnology 6, no. 1 (2021): 32-49.
      2. Cheng, Ronghai, Rui Lai, Chao Peng, Juan Lopez, Zhihong Li, Nathchar Naowarojna, Kelin Li et al. “Implications for an Imidazole-2-yl Carbene Intermediate in the Rhodanase-Catalyzed C–S Bond Formation Reaction of Anaerobic Ergothioneine Biosynthesis.” ACS catalysis 11, no. 6 (2021): 3319-3334.

    • 2020

      1. Cheng, Ronghai, Lian Wu, Rui Lai, Chao Peng, Nathchar Naowarojna, Weiyao Hu, Xinhao Li et al. “Single-step replacement of an unreactive C–H bond by a C–S bond using polysulfide as the direct sulfur source in the anaerobic ergothioneine biosynthesis.” ACS catalysis 10, no. 16 (2020): 8981-8994.

    • 2019

      1. Song, H., Naowarojna, N., Cheng, R., Lopez, J., & Liu, P. (2019). Non-heme iron enzyme-catalyzed complex transformations: endoperoxidation, cyclopropanation, orthoester, oxidative CC and CS bond formation reactions in natural product biosynthesis. Advances in protein chemistry and structural biology, 117, 1-61.
      2. Naowarojna, Nathchar, Seema Irani, Weiyao Hu, Ronghai Cheng, Li Zhang, Xinhao Li, Jiesheng Chen, Yan Jessie Zhang, and Pinghua Liu. “Crystal structure of the ergothioneine sulfoxide synthase from Candidatus Chloracidobacterium thermophilum and structure-guided engineering to modulate its substrate selectivity.” ACS catalysis 9, no. 8 (2019): 6955-6961.
      3. Deng, Qian, Yang Liu, Linyue Chen, Meiling Xu, Nathchar Naowarojna, Norman Lee, Li Chen et al. “Biochemical Characterization of a Multifunctional Mononuclear Nonheme Iron Enzyme (PtlD) in Neopentalenoketolactone Biosynthesis.” Organic letters 21, no. 18 (2019): 7592-7596.
      4. An, Xingda, Nathchar Naowarojna, Pinghua Liu, and Bjorn M. Reinhard. “Hybrid plasmonic photoreactors as visible light-mediated bactericides.” ACS applied materials & interfaces 12, no. 1 (2019): 106-116.

    • 2018

      1. Naowarojna, Nathchar†, Ronghai Cheng†, Li Chen†, Melissa Quill, Meiling Xu, Changming Zhao, and Pinghua Liu. “Mini-Review: Ergothioneine and Ovothiol Biosyntheses, an Unprecedented Trans-Sulfur Strategy in Natural Product Biosynthesis.” Biochemistry (2018).
      2. Chen, Li, Nathchar Naowarojna, Heng Song, Shu Wang, Jiangyun Wang, Zixin Deng, Changming Zhao, and Pinghua Liu. “Use of a Tyrosine Analogue To Modulate the Two Activities of a Nonheme Iron Enzyme OvoA in Ovothiol Biosynthesis, Cysteine Oxidation versus Oxidative C–S Bond Formation.” Journal of the American Chemical Society 140, no. 13 (2018): 4604-4612.
      3. Irani, Seema†, Nathchar Naowarojna†, Yang Tang, Karan R. Kathuria, Shu Wang, Anxhela Dhembi, Norman Lee et al. “Snapshots of CS Cleavage in Egt2 Reveals Substrate Specificity and Reaction Mechanism.” Cell chemical biology 25, no. 5 (2018): 519-529.
      4. Shu-Shan Gao,† Nathchar Naowarojna,† Ronghai Cheng,† Xueting Liu,* and Pinghua Liu,*  “Recent Examples of a-Ketoglutarate-Dependent Mononuclear Non-Haem Iron Enzymes in Natural Product Biosyntheses and Metabolisms. Nat. Prod. Rep., 2018, 35, 792 – 837. †Contributed equally to the work.
      5. R. Zhang, Y. Hong, B. M. Reinhard, Pinghua Liu, Ren Wang, and L. Dal Negro*, “Scalable cylindrical nanotrough networks for Raman-based biosensing”, ACS Appl. Mater. Interface, 2018, 10 (33), 27928–27935
      6. Nathchar Naowarojna,† Pei Huang,† Yujuan Cai, Heng Song, Lian Wu, Ronghai Cheng, Yan Li, Shu Wang, Huijue Lyu, Lixin Zhang, Jiahai Zhou,* Pinghua Liu* “Characterization of the Remaining Steps in Ovothiol A Biosynthetic Pathway: C-S Lyase and Methyltrasferase” Org. Lett 2018, DOI: 10.1021/acs.orglett.8b02332, †Contributed equally to the work.
      7. Wei-chen Chang,* Pinghua Liu,* and Yisong Guo,* “Mechanistic Elucidation of Catalytically Versatile Iron(II) and α-Ketoglutarate Dependent Enzymes: Cases Beyond Hydroxylation”, Inorg. Chem., 2018, in press.
      8. Naowarojna, N.†, Huang, P.†, Cai, Y.†, Song, H., Wu, L., Cheng, R., Li, Y., Wang, S., Lyu, H., Zhang, L. and Zhou, J., 2018. In Vitro Reconstitution of the Remaining Steps in Ovothiol A Biosynthesis: C–S Lyase and Methyltransferase Reactions. Org. lett., 20(17), pp.5427-5430.
      9. Chen, L., Naowarojna, N., Chen, B., Xu, M., Quill, M., Wang, J., … & Liu, P. (2018). Mechanistic studies of a nonheme iron enzyme OvoA in ovothiol biosynthesis using a tyrosine analogue, 2-amino-3-(4-hydroxy-3-(methoxyl) phenyl) propanoic acid (MeOTyr). ACS Catal., 9(1), 253-258.

    • 2017

      1. Pinghua Liu, and Lubin Jiang. “Conditional knockout tools: Application of site-specific incorporation of unnatural amino acid via genetic code expansion in viral and parasite vaccine development.” Synthetic and systems biotechnology1 (2017): 2.
      2. Kathuria, K. R., Irani, S., Liu, P., & Zhang, Y. (2017). Examining the Mechanism of Egt2 in Ergothioneine Biosynthesis.The FASEB Journal, 31(1 Supplement), 606-8.
      3. Qin, L., Chen, C., Chen, L., Xue, R., Ou-Yang, M., Zhou, C., Zhao, S., He, Z., Xia, Y., He, J. and Liu, P., 2017. Worldwide malaria incidence and cancer mortality are inversely associated. Infectious agents and cancer, 12(1), p.14.

    • 2016

      1. Christian G. Roessler, Rakhi Agarwal, Stuart Myers, Anthony Kuczewski, Matthew Cowan, Babak Andi, Annie Héroux, Jan Kern, Rosalie Tran, Ruchira Chatterjee, Guangye Han, Junko Yano, Vittal Yachandra, Anthony Gizzi, Jeffrey B. Bonanno, James D. Love, Steven C. Almo, Eunsun Cho, Heng Song, Pinghua Liu, José F. R. Bachega, Richard C. Garratt, Sammy Datwani, Brent Eaton, Richard Ellson, Lucien P. Ghislain, Lars Majlof, William M. McClintock, Joe Olechno, Richard G. Stearns, Carrie M. Wilmot, Erik T. Yukl, Victor L. Davidson, Aaron S. Brewster, Johan Hattne, Monarin Uervirojnangkoorn, YingssuTsai, Diling Zhu, Roberto Alonso-Mori, Silke Nelsen, Nicholas K. Sauter, Henrik Lemke, Aina Cohen, S. Michael Soltis, Marc Allaire, Alexei S. Soares, & Allen M. Orville, “ Acoustic injectors for drop-on-demand serial femtosecond crystallography”, Structure, 2016, 24 (4),, 631–640
      2. Li Qin, Changzhong Chen, Lili Chen, Ran Xue, Jingping Zheng, Ming Ou-Yang, Susu Gao, Zhengxiang He, Qinyan Li, Xibao Shi, Wanwan Xu, Siting Zhao, Brandon Xia, Jianxing He, Pinghua Liu, Nanshan Zhong,* Xiaoping Chen.* “World-wide epidemiological data analysis: an inverse association between malaria and cancer”, PLos One.2016
      3. James Kirbyb,c,*, Kevin Dietzela,*, Gale Wichmanna,*, Rossana Chanb,c, Eugene Antipova, Nathan Moss, Edward E. K. Baidoo, Peter Jackson, Sara Gaucher, Shayin Gottlieb, Jeremy LaBarge, Tina Mahatdejkul, Kristy Hawkins, Sheela Muley, Jack Newman, Pinghua Liu, Jay D. Keasling and Lishan Zhao, “Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae”, Metab. Eng. In revision.

    • 2015

      1. Wupeng Yan1†, Heng Song2†, Fuhang Song3, Cheng-Hsuan Wu2, Ampon Sae Her2, Yi Pu2,4, Shu Wang2, Catherine E. Costello2,4, Lixin Zhang3*, Pinghua Liu2*, and Yan Zhang1,5* “Endoperoxide Formation Catalyzed by FtmOx1, an α-Ketoglutarate-dependent Mononuclear Non-heme Iron Enzyme”, Nature, 2015, 527, 539–543.
      2. Luwen Ning1#, Guan Wang2#, Zhoufang Li1#, Wen Hu3, 4#, Qingming Hou2, Yin Tong1, Meng Zhang1, Li Qin4, Xiaoping Chen4, Heng-Ye Man2*, Pinghua Liu3*, Jiankui He1*“Quantitative assessment of single-cell sequencing methods using hippocampal neurons”, , 2015; 5: 11415.
      3. Heng Song, Wen Hu, Ampon SaeHer, Rushil Desai, Li Qin,* Xiaoping Chen,* and Pinghua Liu*. “Mechanistic studies of a novel C-S lyase in ergothioneine biosynthesis: the involvement of a sulfenic acid intermediate”, Rep., 2015; 5: 11870

    • 2014

      1. Heng Song, Ampon Sae Her, Fiona Raso, Zhibin Zhen, and Pinghua Liu*,” Cysteine Oxidation Reactions Catalyzed by a Mononuclear Non-heme Iron Enzyme (OvoA) in Ovothiol Biosynthesis. , 2014, 16 (8), 2122–2125
      2. Wen Hu, Heng Song, Ampon SaeHer, Daniel W. Bak, Nathchar Naowarojna, Sean J. Elliott, Li Qin,* Xiaoping Chen,* and Pinghua Liu*. “Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungal Neurospora crassa ergothioneine biosynthetic pathway”, Lett., 2014, 16 (20), 5382–5385

    • 2013

      1. Wei-chen Chang, Mishtu Dey, Pinghua Liu, Steven O. Mansoorabadi, Sung-Ju Moon, Zongbao Zhao, Catherine L. Drennan, and Hung-wen Liu, “An unprecedented 1,2-phosphono migration catalyzed by (S)-2-hydroxypropylphosphonate epoxidase provides evidence for the intermediacy of a cation species in a non-heme mononuclear iron-dependent enzyme-catalyzed reaction”, Nature, 2013, 496, 114 – 118.
      2. Lishan Zhao, Wei-chen Chang, Youli Xiao, and Hung-wen Liu, and Pinghua Liu,* “Isoprenoid Biosynthesis: Mechanistic Studies of the Mevalonate and Deoxyxylulose Phosphate Pathways and Their Biomedical Applications”, Invited review,  Biochem., 2013, 82, 497 – 530.
      3. Heng Song, Maureen Leninger, Norman Lee, and Pinghua Liu*,” Regioselectivity of the oxidative C-S bond formation in ergothioneine and ovothiol biosyntheses”, Lett. 2013, 15, 4854 – 4857.
      4. Wei-chen Chang, Heng Song, and Hung-wen Liu,* and Pinghua Liu,* “Current Development in Isoprenoid Precursor Biosynthesis and Regulation”, Invited review, Opin. Chem. Biol., 2013, 17, 571 -579.

    • 2012

      Kelly Daughtry, Youli Xiao, Deborah Stoner-Ma, Eunsun Cho, Allen M. Orville, Pinghua Liu*, and Karen N. Allen*, “Quaternary Ammonium Oxidative Demethylation: X-ray Crystallographic and on-beam Raman and UV-visible Spectroscopic  Characterization of a Rieske-type Demethylase”, Am. Chem. Soc., 2012, 134, 2823-2834.

    • 2011

      1. Youli Xiao, Wei-chen Chang, Hung-wen Liu*, and Pinghua Liu*, “Study of IspH, a Key Enzyme in the Methylerythritol Phosphate Pathway Using Fluoro-Substituted Substrate Analogues”, 2011, 13, 5912–5915.
      2. Youli Xiao, Debra Rooker, Quincy Young, Caren L. Freel Meyers, Pinghua Liu*. “IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications” ChemBiochem, 2011, 12, 527-530.
      3. Wei-chen Chang, Youli Xiao, Hung-wen Liu,* and Pinghua Liu,* “Mechanistic Studies of an IspH-Catalyzed Reaction: Implications for Substrate Binding and Protonation in the Biosynthesis of Isoprenoids”.  Chem.Int. Ed., 2011, 50, 12304 –12307.

    • 2010

      1. Youli Xiao, Rodney L. Nyland II, Caren L. Freel Meyers, Pinghua Liu*, “Methylerythritol Cyclodiphosphate (MEcPP) in Deoxyxylulose Phosphate Pathway: Synthesis from an Epoxide and Mechanisms” Commun., 2010,  7220 – 7222.

    • 2009

      1. Youli Xiao, Kent Lee, Miranda Machacek,Pinghua Liu*. “Examing the prenyltransferase substrate binding pocket flexibility for isoprenoid profiling”, invited Molecular Biosystems article for a special issue devoted to outstanding young scientists at the chemical- and systems-biology interfaces. 2009, 5(9), 913 – 917.
      2. Youli Xiao, Pinghua Liu*.  “Synthesis of stereo-specific isotopically-labeled substrate analogues of IspH enzyme in the deoxyxylulose phosphate pathway”, Tetrahedron Lett.2009 50, 309-311.
      3. Youli Xiao, Lisa Chu, Yiannis Sanakis,Pinghua Liu*. “Revisiting the IspH Catalytic System in the Deoxyxylulose Phosphate Pathway: Achieving High Activity”, Chem. Soc.  2009, 131(29), 9931 – 9933.
      4. Rodney L. Nyland II, Youli Xiao, Pinghua Liu,* Caren L. Freel Meyers*. “IspG converts an epoxide substrate analog to (E)-4-hydroxy-3-methylbut-2-enyl diphosphate: implications for IspG catalysis in isoprenoid biosynthesis”  Chem. Soc.,2009,131 (49), 17734-17735.
      5. Youli Xiao, Georgia Zahariou, Yiannis Sanakis, and Pinghua Liu*. “IspG Enzyme Activity in the Deoxyxylulose Phosphate Pathway: Roles of the Iron-Sulfur Cluster.”  Biochemistry,2009, 48, 10483 – 10485.

    • 2008

      1. Youli Xiao, Zongbao Zhao, Pinghua Liu*.  “Mechanistic Studies of IspH in the Deoxyxylulose Phosphate Pathway: Heterolytic C-O Bond Cleavage at C4Position”,  Chem. Soc. 2008, 130, 2164-2165.
      2. Youli Xiao,Pinghua Liu*.  “IspH Protein of the Deoxyxylulose Phosphate Pathway: Mechanistic Studies with C1 Deuterium Labelled Substrate and Fluorinated Analogues”,  Int. Ed. 2008, 47, 9722-9725.
      3. Youli Xiao, Kent Lee, Pinghua Liu*.  “Synthesis of the Substrate Candidates for P-methylation Enzyme in Bialaphos Biosynthesis: N-acetyl-demethyl phosphinothricin and N-acetyl-demethyl phosphinothricin tripeptide”, 2008, 10, 5521–5524.

    • 2001~2007

      1. Pinghua Liu, Kazuo Murakami, Takayuki Seki, Xuemei He, Siu-Man Yeung, Tomohisa Kuzuyama, Haruo Seto, and Hung-wen Liu.  “Protein Purification and Function Assignment of the Epoxidase Catalyzing the  Formation of Fosfomycin”,  Chem. Soc. 2001,123, 4619-4620.
      2. Zongbao Zhao*,Pinghua Liu*, Kazuo Murakami, Tomohisa Kuzuyama, Haruo Seto, and Hung-wen Liu. “Mechanistic Studies of HPP Epoxidase: Configuration of the Substrate Governs Its Enzymatic Fate”, * equal contribution;  Int. Ed. 2002, 41, 4529-32.
      3. Pinghua Liu, Aimin Liu, Feng Yan, Matt D. Wolf, John D. Lipscomb, and Hung-wen Liu.“Biochemical and Spectroscopic Studies on (S)-2-Hydroxypropylphosphonic Acid Epoxidase: A Novel Mononuclear Non-heme Iron Enzyme”, Biochemistry 2003, 42, 11577-86.
      4. Pinghua Liu, Mark Mehn, Feng Yan, Zongbao Zhao, Lawrence Que, Jr., and Hung-wen Liu. “Oxygenase Activity in the Self-Hydroxylation of (S)-2-Hydroxypropylphosphonic Acid Epoxidase Involved in Fosfomycin Biosynthesis”,  Chem. Soc.2004, 126, 10306-10312.
      5. Luke J. Higgins, Feng Yan, Pinghua Liu, Hung-wen Liu, Catherine L. Drennan. “Structural Insight into the Biosynthesis of the Antibiotic Fosfomycin by a Mononuclear Iron Enzyme”, Nature, 2005, 437, 838-844.
      6. JoAnne Stubbe, Jiamin Tian, Aimin He, Anthony J. Sinskey, Adam G. Lawrence, and Pinghua Liu. “Non-template dependent polymerization process: polyhydroxyalkanoate synthases as a paradigm”  Biochem. 2005,74, 433-480.
      7. Jiamin Tian, Aimin He, Adam G. Lawrence, Pinghua Liu,Anthony J. Sinskey, and JoAnne Stubbe. “Analysis of Transient Polyhydroxybutyrate Production in Ralstonia eutropha H16 using quantitative Westerns and Electron Microscopy”,  2005, 187, 3825-3832.
      8. Adam G. Lawrence, Joerg Schoenheit, Jiamin Tian, Aimin He, Pinghua Liu, JoAnne Stubbe, and Anthony J. Sinskey. “Transcriptional analysis of Ralstonia eutrophagenes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation”,  Biotech., 2005, 68,  663-672.
      9. Feng Yan, Jeffrey W. Munos, Pinghua Liu,Hung-wen Liu “Biosynthesis of Fosfomycin., Re-examination and Re-confirmation of A Uniqee Fe(II) and NAD(P)H-dependent Epoxidation Reaction”.  Biochemistry 45, 11473-11481, 2006.
      10. Feng Yan, Sung-Ju Moon, Pinghua Liu, Zongbao Zhao, John D. Lipscomb, Aimin Liu, and Hung-wen Liu“Effects of Isotope-enriched Substrates And Substrate Analogues on the Active Site Iron in (S)-2-Hydroxypropylphosphonic Acid Epoxidase (HppE)”. Biochemistry 46 (44): 12628-12638, 2007.

Patent

    1. Pinghua Liu, Heng Song, Wen Hu,”Production of ergothioneine by metabolic engineering (WO 2014100752 A1)”, 2013