Educational Resources

BU Glacios-2 Single Particle Analysis Protocol

Detailed protocol for Glacios-2 single particle analysis screening and data collection using Thermofisher EPU software

20251124_Glacios-2_cryoEM_single_particle_analysis_screening_and_data_collection_protocol

BU Glacios 2 Tomography Protocol

Detailed protocol for Glacios-2 tomography analysis screening and data collection using Thermofisher Tomo software

20251125_BU_Glacios-2_tomography_screening_and_data_collection_protocol

CryoEM 101 & cryoET 101

Basic overview of cryoEM and cryoET with useful diagrams, videos, and images

https://cryoem101.org/

ThermoFisher Cryo-EM University

Comprehensive video series showing the full cryoEM pipeline

https://www.thermofisher.com/us/en/home/electron-microscopy/life-sciences/learning-center/cryo-em-university.html

CryoSPARC Tutorial Videos

In-depth tutorial videos explaining how to use cryoSPARC for processing cryoEM data

https://guide.cryosparc.com/processing-data/tutorial-videos

Caltech | Getting Started in Cryo-EM

Theory, technology, and sample preparation in Cryo-EM

https://cryo-em-course.caltech.edu/

CryoSPARC Discussion Forum

Useful web forum to facilitate discussion on cryoEM data processing using the cryoSPARC software

https://discuss.cryosparc.com/

Other Resources

EPU User Manual v3.8 25-APR-2024

Tomography User Manual v5.19 09-JUL-2024

Falcon4i_Apps_Note_v1.6

Sherpa User Manual 7.13

ThermoFisher_Talos_Arctica_&_Glacios_Training_Packet

Vitrobot User Manual v103261 15-APR-2025