Graded field microscopy

Experimental setup: incoherent white light trans-illuminates a sample in a 6f (unit magnification) imaging line. Partial beam blocks are introduced in the illumination (top) and/or detection (bottom) apertures.

Graded-field microscopy is a general technique for obtaining phase-gradient contrast in biological tissue slices. The technique is based on introducing partial beam blocks in the illumination and detection apertures of a standard white-light widefield transillumination microscope. Depending on the relative aperture sizes, one block produces phase-gradient contrast while the other reduces brightfield background, allowing a full operating range between brightfield and darkfield contrast.

  • K. K. Chu, R. Yi, J. Mertz, “Graded-field autoconfocal microscopy”, Opt. Express 15, 2476-2489 (2007). link
  • R. Yi, K. K. Chu, J. Mertz, “Graded-field microscopy with white light”, Opt. Express 14, 5191-5200 (2006). link