Extended-depth-of-field microscopy

Standard vs. Extended PSF.

Description: We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Significantly increased contrast can be obtained by combining EDOF imaging with targeted illumination. The latter strategy involves delivering illumination only to in-focus structure during the focal sweep, using illumination masks controlled by a fast DMD.

Video-rate EDOF imaging. Conventional EDOF (using a tunable lens) is contrast enhanced by targeted illumination (using a DMD).
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