Year 1

Aim 1: Evaluate the contribution of marketing & contextual factors (place, companionship, affective state, cravings) to poly-tobacco product use.

Aim 2: Develop single cell sequencing and computational methodology to evaluate vascular tobacco product toxicity.

Aim 3: Model trajectories of product use across the life course and their net effects on population level health.

Year 2

Aim 1: Ascertain the individual and environmental factors that drive JUUL use among young adults

Aim 2: Evaluate the impact of JUUL use and product characteristics (nicotine salts) on cardiovascular gene expression patterns and function

Aim 3: Assess tobacco use patterns and transitions within a nationally representative, longitudinal dataset of youth and how implementation of new policies impacts youth and young adult tobacco use