Aim 2: Develop single cell sequencing and computational methodology to evaluate vascular tobacco product toxicity.
Aim 3: Model trajectories of product use across the life course and their net effects on population level health.
Aim 1: Ascertain the individual and environmental factors that drive JUUL use among young adults
Aim 2: Evaluate the impact of JUUL use and product characteristics (nicotine salts) on cardiovascular gene expression patterns and function
Aim 3: Assess tobacco use patterns and transitions within a nationally representative, longitudinal dataset of youth and how implementation of new policies impacts youth and young adult tobacco use