{"id":412,"date":"2022-03-09T14:47:17","date_gmt":"2022-03-09T19:47:17","guid":{"rendered":"https:\/\/sites.bu.edu\/wilsonlab\/?page_id=412"},"modified":"2023-12-29T11:01:59","modified_gmt":"2023-12-29T16:01:59","slug":"interactive-datasets","status":"publish","type":"page","link":"https:\/\/sites.bu.edu\/wilsonlab\/interactive-datasets\/","title":{"rendered":"Interactive Datasets"},"content":{"rendered":"

1) Abo, Merritt et al.
\na) Click here<\/a> to download detailed methods and a table including clinical details of tissue donors
\nb) Click here<\/a> to access an interactive shiny web app allowing interrogation of single cell RNA sequencing from human AATD lung samples (Figures 1-2)
\nc) Click here<\/a> to download a .txt file containing code applied to generate Figures (formatted Seurat object is available with GSE227210)
\nd) Click here<\/a> to download a file containing gene lists used to assign cell identity (Figure 1B)
\ne) Download files containing Hallmark FGSEA with leading edge genes for AT2s<\/a> (used for Figure 2A) or AMs<\/a> (used for Figure 2E)<\/p>\n

2) Werder et al. The American Journal of Human Genetics<\/em>. 2023 doi:10.1016\/j.ajhg.2023.08.017.<\/span>
\nSee the effect of ADGRG6 knockdown on gene expression in iPSC-derived AT2s! SPRING<\/a> or Shiny App<\/a> from Figure 3 (scRNAseq of BU3NGST-CRISPRi iAT2s in 3D +\/- ADGRG6 knockdown)<\/span><\/p>\n

3) Kaserman et al.\u00a0Cell Reports. <\/i>2022\u00a041, 111775.<\/span><\/span>
\na) Click here<\/a> to interact with bulk RNA sequencing from syngeneic, CRISPR-edited PiZZ6 ZZ vs MZ vs MM iHeps from Figure 2
\nb) Click here<\/a> to interact with metabolomics from syngeneic, CRISPR-edited PiZZ6 ZZ vs MZ vs MM iHeps from Figure 3
\nc) Click here<\/a> to interact with scRNAseq data (shiny app of scRNAseq of PiZZ6-ZZ vs PiZZ1-ZZ vs MZ vs MM iHeps)\u00a0from Figure 5
\nd)\u00a0Click here<\/a> to interact with scRNAseq data (SPRING of scRNAseq of PiZZ6-ZZ vs PiZZ1-ZZ vs MZ vs MM iHeps)\u00a0from Figure 5<\/p>\n

4) Werder et al. Sci Adv<\/em>. 2022 Jul 15;15;8(28):eabo6566.
\nClick here<\/a> to see the effect of DSP knockdown on gene expression in iPSC-derived AT2s! SPRING from Figure 4 (scRNAseq of BU3NGST-CRISPRi iAT2s in 3D +\/- DSP knockdown)<\/p>\n

5) Abo et al. JCI Insight.<\/i> 2022 Mar 22;7(6)<\/a>:e155589.
\nClick here<\/a> to see the effect of ALI culture or cigarette smoke exposure on your favorite gene in iPSC-derived AT2s! SPRING from Figure 4 (scRNA-seq of SPC2 iAT2s in 3D, 2D, ALI, and smoke-exposed ALI cultures).<\/span><\/p>\n

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1) Abo, Merritt et al. a) Click here to download detailed methods and a table including clinical details of tissue donors b) Click here to access an interactive shiny web app allowing interrogation of single cell RNA sequencing from human AATD lung samples (Figures 1-2) c) Click here to download a .txt file containing code […]<\/p>\n","protected":false},"author":1896,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/pages\/412"}],"collection":[{"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/users\/1896"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/comments?post=412"}],"version-history":[{"count":21,"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/pages\/412\/revisions"}],"predecessor-version":[{"id":469,"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/pages\/412\/revisions\/469"}],"wp:attachment":[{"href":"https:\/\/sites.bu.edu\/wilsonlab\/wp-json\/wp\/v2\/media?parent=412"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}