{"id":1911,"date":"2022-04-01T13:29:55","date_gmt":"2022-04-01T17:29:55","guid":{"rendered":"https:\/\/sites.bu.edu\/tianlab\/?p=1911"},"modified":"2022-05-05T13:30:40","modified_gmt":"2022-05-05T17:30:40","slug":"cm2-project-receives-nih-r01-from-ninds","status":"publish","type":"post","link":"https:\/\/sites.bu.edu\/tianlab\/2022\/04\/01\/cm2-project-receives-nih-r01-from-ninds\/","title":{"rendered":"CM2 project receives NIH R01 from NINDS!"},"content":{"rendered":"
Computational Miniature Mesoscope for Cortex-wide, Cellular resolution Ca2+ Imaging in Freely Behaving Mice<\/strong><\/a><\/div>\n

 <\/p>\n

ABSTRACT<\/u><\/strong><\/p>\n

Perception and cognition arise from the coordinated activity of large networks of neurons spanning diverse brain areas. Understanding their emergent behavior requires large-scale activity measurements both within and across regions, ideally at single cell resolution. An integrative understanding of brain dynamics requires cellular-scale data across sensory, motor, and executive areas spanning more than a centimeter. In addition, functional interactions between brain areas vary with motivational state and behavioral goals, making data from freely moving animals particularly critical.\u00a0<\/span>Thus, a key goal is the ability to measure activity across the full extent of cortex at cellular resolution as animals engage in complex, cognitively demanding behaviors.\u00a0<\/span><\/u>However, conventional fluorescence microscopy techniques cannot meet the joint requirements of FOV, resolution, and miniaturization. Here,\u00a0<\/span>we propose a Computational Miniature Mesoscope (CM2<\/sup>)<\/strong>\u00a0<\/span>that will enable cortex-wide, cellular resolution Ca2+<\/sup>\u00a0<\/span>imaging in freely behaving mice.\u00a0<\/span><\/strong>The premise is that\u00a0<\/span>computational imaging<\/em>\u00a0<\/span>leverages advanced algorithms to overcome limitations of conventional optics and significantly expand imaging capabilities. In our proof-of-principle system, we demonstrated single-shot 3D imaging across an 8x7mm2<\/sup>\u00a0<\/span>FOV and 7\u00b5m resolution in scattering phantoms (Sci. Adv.<\/em>\u00a0<\/span>2020), and achieved single-cell resolution on histological sections. Our wearable prototype has now demonstrated visualization of sensory-driven neural activity across the 4x4mm2<\/sup>\u00a0<\/span>main olfactory bulb in both\u00a0<\/span>head-fixed<\/em>\u00a0<\/span>and\u00a0<\/span>freely moving<\/em>\u00a0<\/span>mice. In this project we will: (Aim 1<\/strong>) advance CM2<\/sup>\u00a0<\/span>hardware to achieve cortex-wide cellular resolution imaging. We will validate the hardware improvement on both phantoms and\u00a0<\/span>in vivo<\/em>\u00a0<\/span>experiments. (Aim 2<\/strong>) Develop scattering-informed deep learning for fast and robust recovery of neural signals. We will validate the algorithm on\u00a0<\/span>in vivo<\/em>\u00a0<\/span>experiments and benchmark against tabletop 1P and 2P measurements. (Aim 3<\/strong>) Cortex-wide, cellular-resolution Ca2+\u00a0<\/span><\/sup>imaging during social recognition in freely behaving mice. We will use CM2<\/sup>\u00a0<\/span>to investigate the cross-area, network-scale activity dynamics that guide social interactions between familiar partners \u2013 one of the most integrative, multi-sensory, and cognitively demanding forms of neural processing.\u00a0<\/span>IMPACT ON PUBLIC HEALTH<\/strong>: This work will establish powerful enabling technology that greatly expands the scale of activity measurements possible in behaving animals, providing access to a wide range of questions about distributed cortical function. As a focused application, we will test the neural signatures of individual recognition during social behavior. We anticipate that our approach can be extended to a broader range of biological questions such as navigation, short- and long-term memory storage, and can potentially lead to new strategies for characterizing the disruptions in neural function that occur in psychiatric disease and neurodegenerative disorders.<\/p>\n","protected":false},"excerpt":{"rendered":"

Computational Miniature Mesoscope for Cortex-wide, Cellular resolution Ca2+ Imaging in Freely Behaving Mice   ABSTRACT Perception and cognition arise from the coordinated activity of large networks of neurons spanning diverse brain areas. Understanding their emergent behavior requires large-scale activity measurements both within and across regions, ideally at single cell resolution. An integrative understanding of brain […]<\/p>\n","protected":false},"author":12228,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[6],"tags":[],"_links":{"self":[{"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/posts\/1911"}],"collection":[{"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/users\/12228"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/comments?post=1911"}],"version-history":[{"count":2,"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/posts\/1911\/revisions"}],"predecessor-version":[{"id":1913,"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/posts\/1911\/revisions\/1913"}],"wp:attachment":[{"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/media?parent=1911"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/categories?post=1911"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.bu.edu\/tianlab\/wp-json\/wp\/v2\/tags?post=1911"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}