{"id":5,"date":"2012-11-02T15:15:30","date_gmt":"2012-11-02T19:15:30","guid":{"rendered":"https:\/\/sites.bu.edu\/cherrylab\/home\/"},"modified":"2015-05-29T12:20:32","modified_gmt":"2015-05-29T16:20:32","slug":"home","status":"publish","type":"page","link":"https:\/\/sites.bu.edu\/cherrylab\/","title":{"rendered":"Welcome"},"content":{"rendered":"

\"Cherry\"<\/a><\/p>\n

Research Interests:<\/h3>\n

My research and teaching interests concern the relationship between brain and behavior. Studies my lab has conducted on pheromone detection and processing in mice represent a long-standing collaboration with my colleague Dr. Michael Baum <\/a>in the BU Biology Dept.\u00a0 Over the years our labs have published a series of investigations aimed at understanding how the two sexes differentially process socially relevant odors (pheromones), and defining the particular roles of the main and accessory olfactory pathways in pheromone detection.<\/h4>\n

We are using the mouse to examine whether the main olfactory bulb (MOB) detects social odorants by a pathway involving the \u2018vomeronasal amygdala\u2019.\u00a0 We have found that there are direct projections from MOB mitral or tufted (M\/T) cells to the same subnuclei of the medial amygdala that receive direct inputs from the acessory olfactory bulb (AOB).<\/h4>\n

Using anterograde tracing the figures below show demonstrate that the MOB has direct projections to the medial amygdala:<\/h4>\n

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A direct projection from mitral\/tufted (M\/T) cells in the main olfactory bulb (MOB) to the medial amygdala exists, and whether medial amygdala-projecting M\/T cells are activated by volatile urinary odors from conspecifics or a predator (cat). Simultaneous anterograde tracing using Phaseolus vulgaris leucoagglutinin and Fluoro-Ruby placed in the MOB and accessory olfactory bulb (AOB), respectively, revealed that axons of MOB M\/T cells projected to superficial laminae of layer Ia in anterior and posterodorsal subdivisions of the medial amygdala, whereas projection neurons from the AOB sent axons to non-overlapping, deeper layer Ia laminae of the same subdivisions. Placement of the retrograde tracer cholera toxin B into the medial amygdala labeled M\/T cells that were concentrated in the ventral MOB.<\/p>\n

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Research Interest<\/span>: Flattened Cortex Preparation.<\/h2>\n

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A whole-mount, flattened cortex preparation was developed to compare profiles of axonal projections from main olfactory bulb (MOB) and accessory olfactory bulb (AOB) mitral and tufted (M\/T) cells. After injections of the anterograde tracer, Phaseolus vulgaris leucoagglutinin, mapping of labeled axons using a Neurolucida system showed that M\/T cells in the AOB sent axons primarily to the medial and posterior lateral cortical amygdala, with minimal branching into the piriform cortex.<\/h3>\n

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\"Picture1\"<\/a> (a)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 (b)<\/strong><\/p>\n

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(a) Efferent projections from the anterior medial amygdala (green) and posterior medial amygdala (red) passing through the posterolateral stria terminalis on their way to hypothalamic and ventral striatal targets.\u00a0(b) Top: Neuron coexpressing Fos protein (black) and the retrograde tracer, CTB (brown) in the Basolateral Amydgala of mouse\u00a0 given an injection of CTB into the medial olfactory tubercle and subsequently exposed to opposite sex odors.\u00a0 Bottom: Neuron expressing only CTB projecting to the medial olfactory tubercle.<\/h3>\n

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Another area\u00a0of Interest<\/span>: Optogenetics<\/h2>\n

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Photostimulation of an arbitrarily chosen ensemble of protocadherin-21\u00a0(PCDH-21 \u00a0+\/+) cells\u00a0expressing ChR2 in Accessory Olfactory Bulb (AOB) mitral cell neurons, excite\u00a0primary neurons\u00a0in Medial Amygdala (MeA)\u00a0during 5 second exposure to 473nm blue LED light.<\/h3>\n

\"Picture1\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Research Interests: My research and teaching interests concern the relationship between brain and behavior. Studies my lab has conducted on pheromone detection and processing in mice represent a long-standing collaboration with my colleague Dr. Michael Baum in the BU Biology Dept.\u00a0 Over the years our labs have published a series of investigations aimed at understanding […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"page-templates\/no-sidebars.php","meta":[],"_links":{"self":[{"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/pages\/5"}],"collection":[{"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/comments?post=5"}],"version-history":[{"count":50,"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/pages\/5\/revisions"}],"predecessor-version":[{"id":239,"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/pages\/5\/revisions\/239"}],"wp:attachment":[{"href":"https:\/\/sites.bu.edu\/cherrylab\/wp-json\/wp\/v2\/media?parent=5"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}