{"id":200,"date":"2019-07-05T15:41:08","date_gmt":"2019-07-05T19:41:08","guid":{"rendered":"https:\/\/sites.bu.edu\/biomicroscopy\/?page_id=200"},"modified":"2019-07-15T09:44:07","modified_gmt":"2019-07-15T13:44:07","slug":"gfm","status":"publish","type":"page","link":"https:\/\/sites.bu.edu\/biomicroscopy\/research\/gfm\/","title":{"rendered":"Graded field microscopy"},"content":{"rendered":"<figure id=\"attachment34\" aria-describedby=\"caption-attachment34\" style=\"width: 444px\" class=\"wp-caption alignright\"><img loading=\"lazy\" src=\"\/biomicroscopy\/files\/2019\/06\/Graded-field-microscopy-setup.jpg\" alt=\"\" class=\"wp-image-34 size-full\" width=\"434\" height=\"657\" srcset=\"https:\/\/sites.bu.edu\/biomicroscopy\/files\/2019\/06\/Graded-field-microscopy-setup.jpg 434w, https:\/\/sites.bu.edu\/biomicroscopy\/files\/2019\/06\/Graded-field-microscopy-setup-420x636.jpg 420w\" sizes=\"(max-width: 434px) 100vw, 434px\" \/><figcaption id=\"caption-attachment34\" class=\"wp-caption-text\">Experimental setup: incoherent white light trans-illuminates a sample in a 6f (unit magnification) imaging line. Partial beam blocks are introduced in the illumination (top) and\/or detection (bottom) apertures.<\/figcaption><\/figure>\n<p>Graded-field microscopy is a general technique for obtaining phase-gradient contrast in biological tissue slices. The technique is based on introducing partial beam blocks in the illumination and detection apertures of a standard white-light widefield transillumination microscope. Depending on the relative aperture sizes, one block produces phase-gradient contrast while the other reduces brightfield background, allowing a full operating range between brightfield and darkfield contrast.<\/p>\n<ul>\n<li>K. K. Chu, R. Yi, J. Mertz, \u201cGraded-field autoconfocal microscopy\u201d, Opt. Express 15, 2476-2489 (2007). <a href=\"https:\/\/www.osapublishing.org\/oe\/abstract.cfm?uri=oe-15-5-2476\">link<\/a><\/li>\n<li>R. Yi, K. K. Chu, J. Mertz, \u201cGraded-field microscopy with white light\u201d, Opt. Express 14, 5191-5200 (2006). <a href=\"https:\/\/www.osapublishing.org\/oe\/abstract.cfm?uri=oe-14-12-5191\">link<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Graded-field microscopy is a general technique for obtaining phase-gradient contrast in biological tissue slices. The technique is based on introducing partial beam blocks in the illumination and detection apertures of a standard white-light widefield transillumination microscope. Depending on the relative aperture sizes, one block produces phase-gradient contrast while the other reduces brightfield background, allowing a [&hellip;]<\/p>\n","protected":false},"author":16427,"featured_media":0,"parent":98,"menu_order":27,"comment_status":"closed","ping_status":"closed","template":"page-templates\/profiles.php","meta":[],"_links":{"self":[{"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/pages\/200"}],"collection":[{"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/users\/16427"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/comments?post=200"}],"version-history":[{"count":5,"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/pages\/200\/revisions"}],"predecessor-version":[{"id":460,"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/pages\/200\/revisions\/460"}],"up":[{"embeddable":true,"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/pages\/98"}],"wp:attachment":[{"href":"https:\/\/sites.bu.edu\/biomicroscopy\/wp-json\/wp\/v2\/media?parent=200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}