Research Movies

Neil is featured on BU Research for cancer research, and here is a time-lapse video of everyone working hard in lab!

 

Movie 1. Live-cell imaging of FACS-sorted G1 diploid RPE-1 FUCCI cells following 16 h of exposure to DCB. Upon attachment, cells were transfected with scrambled siRNA and imaged for ~48 hr. 

 

Movie 2. Live-cell imaging of FACS-sorted G1 binucleated tetraploid RPE-1 FUCCI cells following 16 h of exposure to DCB. Upon attachment, cells were transfected with scrambled siRNA and imaged for ~48 hr.

 

Movie 3. Live-cell imaging of FACS-sorted G1 binucleated tetraploid RPE-1 FUCCI following 16 h of exposure to DCB. Upon attachment, cells were transfected with p53 siRNA and imaged for ~48 hr.

 

Movie 4. Live-cell imaging of a U20S cells expressing H2B-Kaede. The labeled-chromosome in the micronucleus is converted from green-fluorescent to red-fluorescent with a pulse of UV light.  The labeled chromosome is then tracked through the cell cycle and can be seen reincorporating into a primary nucleus following mitosis.

 

Movie 5. A cancer cell with H2B-GFP labeled chromosomes undergoing multipolar cell division. All three daughter cells die in the next cell cycle.

 

Movie 6. A tetraploid cell expressing H2B-RFP and GFP-centrin undergoing mitosis. The supernumerary centrosomes cluster into two poles, enabling a bipolar cell division. However, one chromosome bi-orients between two clustered centrosomes, ultimately leading to a chromosome missegregation event at anaphase.  

 

Movie 7. How extra centrosome promote chromosome missegregation and aneuploidy.

 

Movie 8. Colon cancer cells dividing over 3 days.