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[(enginee)10.8(r)-8.8(ed)-346.3(c)10.8(o)-10.2(nstruct)10.8(s)-357.7(b)10.8(y)-352.1(tight)10.8(l)-12.2(y)-341.4(c)-1.1(ontrolling)-347.7(liga)10.8(n)-11.1(d)-340(p)-3.2(rese)10.8(n)-11.5(-)]TJ
0 -1.2266 TD
[(ta)10.7(t)-8.2(i).8(on)-334.1(in)-334.1(tissue)-330.6(engineerin)-5.3(g)-328.8(s)-3.3(c)10.7(a)-7.1(ff)10.7(o)-11.5(l).8(d)-327.4(m).5(ate)10.7(r)-8.9(ials.)]TJ
1.1128 -1.2203 TD
0 Tc
[(In)-176.2(thi)17.9(s)-184.7(wor)13.9(k,)-171.7(ang)11.7(io)12.7(gen)11.3(ic)-173.5(cue)12.1(s)-178.4(were)-166.5(loc)13.4(ali)12.4(ze)13.2(d)-180(prec)17.7(ise)14.2(ly)-178.7(on)-170.6(the)]TJ
-1.1128 -1.2203 TD
[(sur)15.6(fac)13.4(e)-372.9(o)0(f)-370(P)0(E)14.6(G)-371.8(hyd)14.5(roge)17.5(ls)-365.3(by)-369.2(usi)12.8(ng)-361.9(LSL)14.5(.)-376(Pat)17.6(ter)11.6(ni)12(ng)-368.2(the)-364.2(pre-)]TJ
0 -1.2266 TD
[(sen)14.4(tat)12.2(ion)-281.6(of)-287.8(bioc)15.3(hem)11.8(ica)16.6(l)-294.4(s)0(i)14.1(gna)11.7(ls)-289.4(to)-287.8(be)-282.5(hig)14(hly)-283.2(loc)13.4(ali)12.4(zed)-280.6(all)12.4(ows)]TJ
0 -1.2203 TD
[(con)13.5(tro)15(l)-332.3(o)0(f)-325.7(the)-319.9(geo)11.9(met)14.7(ry)-327.6(of)-325.7(the)-319.9(cap)12.5(ill)14.7(ar)12.4(y)-333.2(b)0(e)14.7(d)-331.8(and)-322(als)14.6(o)-331.6(s)0(h)14.3(oul)11.3(d)]TJ
T*
[(disc)18.2(our)14.1(age)-294.7(unc)14.9(ont)12.3(rol)12(led)-291(ang)11.7(io)12.7(gen)11.3(es)11.4(is)-302(in)-297.8(und)17.5(esi)14.2(red)-294.4(loc)13.4(ati)15.5(ons)11.5(.)]TJ
0 -1.2266 TD
[(The)-309.8(pre)17(sen)14.4(ted)-306.9(use)-306.1(of)-313.1(mat)15.1(rix)12.6(-bo)11.3(und)-304.9(VEG)14.4(F)]TJ
6.7246 0 0 5.9768 227.2251 384.5479 Tm
(165)Tj
8.9663 0 0 8.9663 240.0377 386.0219 Tm
[(in)-310.4(pat)11.3(te)12.3(rned)-304.2(lo-)]TJ
-19.8539 -1.2203 TD
[(cat)13.4(ion)15.6(s)-336.4(wit)13.3(hin)-326.4(bio)14.6(com)13(pat)11.3(ibl)13.8(e)-335(pol)17.6(ymer)-325(hyd)14.5(rog)17.4(els)-327.2(is)-333.6(hyp)16.1(oth)12.3(e-)]TJ
T*
[(siz)14.6(ed)-192.6(to)-199.3(pro)14.1(mo)12.3(te)-196.4(end)12.7(oth)12.3(eli)18.3(al)-192.7(tub)12.7(ulo)11.3(gen)11.3(es)11.4(is,)-191.1(the)15.2(re)12(by)-198.5(lea)16(di)12.4(ng)-197.5(to)]TJ
T*
[(hig)14(hly)-365.4(orga)17.9(ni)12.1(zed)-369.1(ve)12(ssel)-360.1(form)17.5(ati)15.5(on.)-369.5(Whi)13(le)-370.2(re)12(stri)19.3(ctio)12.9(n)-370.1(o)0(f)-370(cell)12.7()]TJ
0 -1.2266 TD
[(mat)15.1(eri)14.8(al)-433(in)12(ter)11.6(act)13.4(ion)15.6(s)-437.6(coul)18.3(d)-439.3(b)0(e)-427.9(used)-422.9(to)-433.2(di)12.4(rec)12.8(t)-436.7(mic)12.3(rov)14.8(asc)12.5(ul)14.1(ar)]TJ
0 -1.2203 TD
[(for)15(mat)15.1(ion)-471.3(and)-473.8(ana)16.6(sto)14.4(mosi)20.1(s)-481.9(o)0(n)-474.1(the)-471.6(sur)15.6(fac)13.4(e)-480.4(o)0(f)-477.5(bio)14.6(mat)15.1(er)12(ial)12.4(s,)]TJ
T*
[(two)14.1(-di)12(men)11.8(si)14.1(ona)13.3(l)-345(pat)17.6(ter)11.6(ni)12(ng)-336.6(als)14.6(o)-344.2(a)0(c)13.9(t)0(s)-336.9(a)0(s)-335.9(a)-340.9(fou)14.4(nda)13.1(tio)12.2(n)-338.5(for)-332.7(fu-)]TJ
0 -1.2266 TD
[(ture)-280.6(stu)15.8(die)12.5(s)-292.2(i)0(n)-291.5(thr)14.4(ee)-290.6(di)12.4(men)11.8(sio)11.4(ns)14.3(.)-293.8(Thi)15.4(s)-298.5(w)0(o)14.6(r)0(k)-289.6(int)11.6(rod)12.5(uc)12(es)-292.1(a)-290.4(s)0(y)13.2(s)0(-)]TJ
0 -1.2203 TD
[(tem)-472.1(that)-471.7(pro)14.1(mot)11.8(es)-475.4(and)-473.8(spat)16.3(ial)12.4(ly)-475.8(con)13.5(tro)15(ls)-479.1(the)-478(for)15(mat)15.1(ion)-477.6(of)]TJ
T*
[(end)12.7(oth)12.3(eli)12(al)-331.8(tub)12.7(ule)14.2(s)-342.8(b)0(y)-337.6(pat)11.3(ter)11.6(ni)12.1(ng)-342.9(the)-332.5(lo)12.7(cali)19.4(zat)13.2(ion)-338.5(of)-338.4(in)12.1(teg)14.2(rin)]TJ
0 -1.2266 TD
[(lig)13.8(and)13.1(s)-273.2(and)-265.1(grow)15.8(th)-269.4(fac)13.4(tor)15(s)-279.5(o)0(n)-271.7(the)-263(surf)15.1(ace)-264.3(of)-275.1(PE)14.6(G)-277(hyd)14.5(roge)17.5(ls.)]TJ
/F4 1 Tf
0 -3.054 TD
[(Materials)-332.7(and)-338.2(Methods)]TJ
/F10 1 Tf
0 -1.8336 TD
[(Cell)-336.3(culture)]TJ
/F3 1 Tf
1.1128 -1.8336 TD
-.0196 Tc
[(Human)-263.3(u)-2(mbilical)-269.7(vein)-269.6(e)5.8(n)-4(dothelial)-263.4(c).1(ells)-267.5(\(HUVEC;)-269.1(C)-.8(a)6.2(m)-4.5(brex/)]TJ
-1.1128 -1.2203 TD
0 Tc
[(Lonz)-8.7(a\))-771.4(were)-773.5(mai)-6.9(ntaine)-10.2(d)-768.1(i)0(n)-778.3(VEGF-fr)-10.6(ee)-771.2(endo)-9.1(thelial)-778.9(cell)]TJ
T*
[(growt)-10(h)-793.7(medium)-9.2(-2)-796.7(\(Camb)-8.5(rex/Lonza\))-804.8(conta)-11.7(ining)-796(hum)-8.6(an)]TJ
0 -1.2266 TD
[(FGF-)-8.1(B,)-322.1(human)-327.7(epidermal)-331.5(growth)-329.5(factor,)-328.6(ins)-8.2(ulin-lik)-11.1(e)-322.4(growth)]TJ
0 -1.2203 TD
[(factor)-243.5(\(R)]TJ
6.7246 0 0 5.9768 95.5842 176.3149 Tm
(3)Tj
8.9663 0 0 8.9663 98.9291 172.3464 Tm
[(-IGF-1)-12.1(\),)-231(hepa)-10.5(rin,)-238.2(hydroco)-10(rtisone,)-242.6(ascorbi)-11.5(c)-233.2(acid,)-235.9(GA-)]TJ
-4.1162 -1.2203 TD
[(1000)-491.2(\(gentamici)-12.1(n,)-486.8(amphote)-11.3(ricin-B\),)-491.5(2%)-488(fetal)-490.7(bov)-7.9(ine)-487.4(serum)]TJ
0 -1.2266 TD
[(\(Bulle)-11.8(tkit,)-271(Lon)-9.2(za\),)-274.3(1)-163.9(U/m)-8.3(L)-268.6(penici)-11(llin,)-276.1(1)]TJ
/F9 1 Tf
17.173 0 TD
(m)Tj
/F3 1 Tf
.5564 0 TD
[(g/mL)-273.5(streptomyc)-14.6(in,)]TJ
-17.7294 -1.2203 TD
[(and)-486.4(2)-163.9(m)0(M)-483.2(L-glutam)-10.3(ine)-481(\(Si)-8.8(gma\).)-485.4(Cells)-482.7(were)-489(maintain)-10.9(ed)-477.1(at)]TJ
T*
(37)Tj
/F9 1 Tf
1.0053 0 TD
(8)Tj
/F3 1 Tf
.3857 0 TD
[(C)-253.1(i)0(n)-259.8(a)-252.4(5%)-254.1(CO)]TJ
6.7246 0 0 5.9768 127.4456 126.9921 Tm
(2)Tj
8.9663 0 0 8.9663 133.1149 128.5228 Tm
[(enviro)-11(nment)-257.6(and)-258.8(use)-8.9(d)-255.9(between)-262.5(passa)-9.3(ges)-258.5(3)]TJ
-7.9289 -1.2267 TD
[(and)-334.7(6.)]TJ
/F10 1 Tf
0 -2.4406 TD
[(Preparation)-339(and)-329.4(purication)-336.9(of)-330.4(PEGDA)]TJ
/F3 1 Tf
1.1128 -1.8336 TD
[(P)-7.7(E)-9.3(G)-11.4(D)-14(A)-494.6(w)-14.3(as)-506.7(p)-14(r)-7(e)-12.5(p)-7.7(a)-12.2(r)-7(e)-12.5(d)-502.5(by)-508.3(r)-7(e)-12.5(ac)-17.8(t)-13.1(i)-9.9(n)-9.7(g)-497.6(P)-14(E)-9.3(G)-498.3(\()-13.1(Fl)-14.3(u)-14(k)-10.7(a/)-21.5(S)-11.1(i)-9.9(gm)-14.6(a)-12.2(;)]TJ
-1.1128 -1.2203 TD
.0114 Tc
(MW)Tj
/F11 1 Tf
2.1245 0 TD
0 Tc
()Tj
/F3 1 Tf
.9358 0 TD
.0107 Tc
[(600)10.7(0)-570(D)-3.3(a\))-565.2(with)-568.1(a)10.7(c)-7.1(rylo)-4.7(yl)-561.9(chloride)-564.6(\(Sigma)10.7(\))-577.3(i).8(n)-568.1(a)-564.2(1)10.7(:)-10.7(4)]TJ
-3.0603 -1.2267 TD
.0104 Tc
[(molar)-420.3(r)-2.9(a)10.4(t)-8.5(io)-422.3(in)-422.9(anhydrous)-427.2(d)1.1(ichloromet)10.4(h)-6.1(a)-1.8(ne)-425.8(\()10.4(D)-10.4(C)10.4(M)-7.5(;)-422.5(S)-.7(igma\))]TJ
27.9978 74.5598 TD
0 Tc
[(c)-11.9(o)-9.1(n)-16(t)0(a)-18.9(i)-9.9(n)-9.7(i)-9.9(n)-9.7(g)-586.1(tr)-20.1(i)-9.9(e)-12.5(th)-16.5(y)-10.7(l)-585.2(am)-16(i)-9.9(n)-16(e)-581.6(\()0(S)-17.9(i)-9.9(g)-10.7(m)-10.2(a)-12.2(\))-582.2(a)-12.2(t)-582.2(a)-581.2(1)-12.2(:)-9.2(2)-581.2(\(P)-20.8(E)-9.3(G)-11.4(:)-9.2(t)-13.1(r)-7(i)-9.9(e)-12.5(t)0(h)-22.8(y)-10.7(l)]TJ
0 -1.2203 TD
.0112 Tc
[(amine\))-311.7(m)11.2(o)-8.1(la)11.2(r)-317.8(r)4.2(atio)-307.7(a)11.2(t)-317.6(25)]TJ
/F9 1 Tf
10.8944 0 TD
0 Tc
(8)Tj
/F3 1 Tf
.3983 0 TD
.3179 Tc
[(Cu)303.9(n)308.2(d)308.6(e)305.4(r)1.1(a)305.7(r)310.9(g)307.2(o)308.8(no)302.5(v)310.8(e)305.4(r)310.9(n)301.9(i)308(g)317.9(h)297.5(t)317.9(.)-8(P)303.9(E)308.6(G)306.5(D)310.2(A)]TJ
-11.2927 -1.2266 TD
0 Tc
[(u)-14(n)-9.7(d)-9.3(e)-12.5(r)-7(w)-14.3(e)-12.5(n)-9.7(t)-683.3(p)-7.7(u)-14(r)-7(i)-9.9()-15.7(ca)-17.8(t)-13.1(i)-9.9(o)-9.1(n)-686.3(by)-691.7(p)-7.7(h)-16(as)-19.8(e)-682.8(s)-7.6(e)-12.5(p)-14(ar)-19.2(at)-19(i)-9.8(o)-9.1(n)-686.3(u)-14(s)-7.6(i)-9.9(n)-9.7(g)-687.3(2)-176.6(M)]TJ
0 -1.2203 TD
(K)Tj
6.7246 0 0 5.9768 319.6346 691.4266 Tm
(2)Tj
8.9663 0 0 8.9663 323.0928 692.9006 Tm
(CO)Tj
6.7246 0 0 5.9768 336.5857 691.4266 Tm
(3)Tj
8.9663 0 0 8.9663 340.044 692.9006 Tm
.0112 Tc
[(.)-402.7(T)1.9(he)-399.7(orga)11.2(n)-10.7(i)1.3(c)-399.1(p)3.5(hase)11.2(,)-408.9(w)3.3(hich)-403.2(c)11.2(o)-3.5(n)-4.8(t)11.2(a)-7.7(i)1.3(ned)-396.5(P)-2.8(EGDA,)-396.4(w)3.3(as)]TJ
-3.0097 -1.2203 TD
.0104 Tc
[(d)-5.3(r)3.4(ied)-466.8(u)2.7(sing)-468.2(anhydro)-5(u)2.7(s)-465.1(M)-1(gSO)]TJ
6.7246 0 0 5.9768 437.8959 680.4282 Tm
0 Tc
(4)Tj
8.9663 0 0 8.9663 445.5495 681.9589 Tm
[(a)-12.2(n)-9.7(d)-477.2()-9.3(l)-9.9(t)-13.1(er)-19.6(e)-12.5(d)-9.3(.)-477.1(P)-7.7(E)-9.3(G)-11.4(D)-14(A)-475.6(w)-7.9(a)-12.2(s)]TJ
-14.7766 -1.2266 TD
.0109 Tc
[()-4.8(r)3.9(st)-425.8(precipita)10.9(t)-8.1(ed)-428.4(in)-428.8(diet)10.9(h)-5.6(y).2(l)-428.9(e)-1.6(the)10.9(r)-8.7(,)-428.3(t)-2.2(he)10.9(n)-435()-4.7(lt)10.9(e)-8.4(r)3.9(ed,)-428.3(a)-1.2(nd)-428.4(drie)10.9(d)]TJ
0 -1.2203 TD
.0143 Tc
[(ov)7.2(er)7.3(ni)4.4(gh)4.6(t)-523.6(a)2.1(n)4.6(d)-526.1(un)4.6(d)5(e)1.8(r)-523.8(v).9(ac)14.3(uu)6.6(m)4.1(.)]TJ
6.7246 0 0 5.9768 445.2093 663.9872 Tm
0 Tc
(1)Tj
8.9663 0 0 8.9663 448.6676 660.0187 Tm
[(H)-12.6(y)-10.7(d)-9.3(r)-13.3(o)-9.1(g)-10.7(en)-553.4(Nu)-20.3(c)-11.9(l)-9.9(ea)-18.4(r)-544.5(M)0(a)-17.3(g)-10.7(-)]TJ
-15.1244 -1.2203 TD
[(n)-16(e)0(t)-19.3(i)-9.9(c)-302.8(R)-10.6(e)-12.5(s)-7.6(o)-9.1(n)-16(an)-15.5(c)-11.9(e)-303.4(\()]TJ
6.7246 0 0 5.9768 382.9038 653.0455 Tm
(1)Tj
8.9663 0 0 8.9663 386.3621 649.077 Tm
.0115 Tc
[(H)11.5(-)-7.9(NMR\))-286.1(chara)11.5(c)-6.3(te)11.5(r)-8.1(i)1.6(za)11.5(t)-7.5(i)1.6(on)-289.1(of)-292.5(PEGDA)-293.4(w)3.6(as)]TJ
-8.1755 -1.2266 TD
0 Tc
[(u)-14(s)-7.6(e)-12.5(d)-534.1(to)-540.7(c)-11.9(o)-9.1(n)-9.7()-9.3(r)-13.4(m)-528.7(a)-12.2(c)-11.9(r)-7(y)-10.7(l)-9.9(a)-12.1(ti)-16.7(o)-15.4(n)-528.2(\()-13.1(A)-7.7(v)-13.4(a)0(n)-21.9(c)0(e)-536.6(4)-12.2(0)-12.2(0)-176.6(Hz)-18.5(;)-534(B)-9.3(r)-7(u)-14(k)-10.7(a)0(\))-537.4(w)-14.3(i)-9.9(t)0(h)]TJ
0 -1.2203 TD
(D)Tj
6.7246 0 0 5.9768 320.0881 625.6628 Tm
(2)Tj
8.9663 0 0 8.9663 323.5464 627.1368 Tm
.0118 Tc
[(O)-231.5(a)-.4(s)-236.1(a)-234.3(solv)4.7(ent.)-231.4(PEGD)4.1(A)-236.2(w)-2.5(a)11.8(s)-241.9(st)11.8(ored)-231.5(under)-235.5(a)11.8(r)-7.4(g)1.1(on)-231.9(at)]TJ
/F11 1 Tf
22.3452 0 TD
0 Tc
()Tj
/F3 1 Tf
.7714 0 TD
.0122 Tc
(20)Tj
/F9 1 Tf
1.018 0 TD
0 Tc
(8)Tj
/F3 1 Tf
.3983 0 TD
(C.)Tj
/F10 1 Tf
-25.7026 -2.4406 TD
[(Preparation)-339(and)-335.7(purication)]TJ
0 -1.2266 TD
[(of)-336.7(acryl-PEG-succinimidyl)-335.3(carbonate)]TJ
/F3 1 Tf
1.1128 -1.8336 TD
[(Acryl-PEG-succinimidyl)-492.4(carbonate)-484.2(\(PEG-SMC\))-482.5(was)-483(syn-)]TJ
-1.1128 -1.2203 TD
[(thesized)-580.1(by)-571.6(reacting)-573.9(PEG)-572.2(\(Fluka/Sigma;)-573.2(MW)]TJ
/F11 1 Tf
21.5675 0 TD
()Tj
/F3 1 Tf
.9358 0 TD
[(3400)-573.4(Da\))]TJ
-22.5032 -1.2203 TD
[(with)-369.4(1.5)-356(molar)-364.5(excess)-364.3(Ag)]TJ
6.7246 0 0 5.9768 414.6519 554.3998 Tm
(2)Tj
8.9663 0 0 8.9663 418.0534 555.9305 Tm
[(O)-357.1(\(Sigma\),)-367.8(1.1)-362.3(molar)-358.2(excess)-364.3(acry-)]TJ
-11.71 -1.2266 TD
[(loyl)-558(chloride)-559.9(\(Sigma\),)-557.5(and)-556(0.3)-552(molar)-554.2(ratio)-557.1(KI)-555(\(Sigma\))-560.9(in)]TJ
0 -1.2203 TD
[(anhydrous)-683.8(dichloromethane)-680.7(\(DCM;)-680.3(Sigma\))-674.2(overnight)-683.2(at)]TJ
T*
(0)Tj
/F9 1 Tf
.5058 0 TD
(8)Tj
/F3 1 Tf
.3794 0 TD
(C4)Tj
/F9 1 Tf
1.7135 0 TD
(8)Tj
/F3 1 Tf
.3857 0 TD
[(C)-430.1(t)0(o)-439.5(produce)-438.3(monoacrylated)-440.5(PEG.)-436(Silver)-436.8(oxide)-435(was)]TJ
-2.9844 -1.2266 TD
[(removed)-352.3(from)-349.3(the)-351.5(resulting)-347.4(solution)-354.3(via)-345.2(ltration)-352.5(using)-349.1(Ce-)]TJ
0 -1.2203 TD
[(lite)-696.6(521)-694.1(\(Spectrum)-695.1(Chemical)-696.1(Manufacturing)-692.7(Corp\).)-697.6(The)]TJ
T*
[(product)-318.8(was)-312.3(dried)-307.3(via)-313.6(Rotovap,)-312(then)-317(dissolved)-310(in)-310.4(deionized)]TJ
T*
[(water)-293.1(\(DI)-288.5(H)]TJ
6.7246 0 0 5.9768 360.907 477.6943 Tm
(2)Tj
8.9663 0 0 8.9663 364.3085 479.2251 Tm
[(O\),)-284.6(and)-290.4(the)-288.3(pH)-292.2(was)-287(titrated)-292.6(to)-287.8(3)-290.4(with)-287.2(HCl.)-291.1(This)]TJ
-5.7159 -1.2266 TD
[(solution)-392.2(was)-381.8(heated)-385.5(at)-379.4(35)]TJ
/F9 1 Tf
11.5962 0 TD
(8)Tj
/F3 1 Tf
.3857 0 TD
[(C)-379.5(for)-383.3(1)-170.2(h)0(,)-379.4(and)-385.3(activated)-385.8(charcoal)]TJ
-11.9819 -1.2203 TD
[(\(Fisher\))-406.2(was)-400.8(added)-400.4(for)-402.3(iodine)-401.8(removal,)-406(followed)-403.3(by)-400.9(subse-)]TJ
T*
[(quent)-363.6(ltration)-358.8(using)-355.4(Celite)-361.6(521.)-355.5(Sodium)-360.8(chloride)-357.5(\(NaCl\))-358.2(in)]TJ
0 -1.2266 TD
[(DCM)-240.6(was)-230.1(added,)-239(followed)-238.9(by)-230.1(DCM)-240.6(extraction.)-236.4(Hydrochloric)]TJ
0 -1.2203 TD
[(acid)-422.6(and)-410.6(chloride)-420.8(ions)-422(were)-413.1(removed)-421.9(through)-421.2(phase)-416.5(sepa-)]TJ
T*
[(ration)-541.5(utilizing)-541.7(2)-170.2(M)-536.2(K)]TJ
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T*
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T*
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T*
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