New paper led by students Will Shain (now at Draper) and Nic Vickers, improving on EDOF microscopy technique by adding axial location. Applications in bacterial tracking and neural imaging are described.
Sunday, January 28, 2018, 8-11PM at the Thirsty Bear, 661 Howard St. San Francisco, CA
Boston University has won an award from the National Science Foundation(NSF) to create a multi-institution Engineering Research Center (ERC), with the goal of synthesizing personalized heart tissue for clinical use.
The Bifano Lab will will direct imaging and optical engineering thrusts in this project.
Paper in Optics Letters:
We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
EpiPens need to be kept within narrow temperature limits. They cannot be refrigerated, nor are they allowed to exceed 86°F. This summer we developed a low-cost, do-it-yourself solution for keeping EpiPens cool on summer outings. A short YouTube video highlights the results.
Matched-Filter Compressive Imaging using a Deformable Mirror for Label-Free Flow Cytometry
William Shain, Bennett Goldberg, Thomas Bifano, and Jerome Mertz
Abstract: We distinguish geometric cell structures using a deformable mirror for high-throughput, label-free flow cytometry, overcoming approaches that can result in undesirable chemical modifications or low throughput. Extended abstract
Novel Deconvolution Kernel for Extended Depth-of-Field Microscopy with a High-Speed Deformable Mirror
William Shain, Nicholas Vickers, Bennett Goldberg, Thomas Bifano, and Jerome Mertz
Abstract: A deformable mirror is used to scan the focal plane during the camera exposure, obtaining extended depth-of-field. A deconvolution kernel is approximated for a given scan depth and used to de-blur the image. Extended abstract
The Photonics Center hosted the 20th Annual Photonics Center Symposium on Optics for Cancer Imaging.
The Photonics Center was awarded a NSF National Research Traineeship (NRT) by the National Science Foundation. The NRT: Understanding the Brain: Neurophotonics, is led by Professor Thomas Bifano. This is a university-wide community of doctoral students in biology, neuroscience, biophysics, biomedical, and mechanical engineering with focused professional development and graduate training in neurophotonics. Learn more about the program.