Author: Thomas G Bifano

Extended depth-of-field microscopy with a high-speed deformable mirror

Paper in Optics Letters: We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits […]

Summer design project: Keep EpiPens cool

EpiPens need to be kept within narrow temperature limits. They cannot be refrigerated, nor are they allowed to exceed 86°F. This summer we developed a low-cost, do-it-yourself solution for keeping EpiPens cool on summer outings. A short YouTube video highlights the results.

Will Shain presents two papers at OSA COSI Conference

Matched-Filter Compressive Imaging using a Deformable Mirror for Label-Free Flow Cytometry William Shain, Bennett Goldberg, Thomas Bifano, and Jerome Mertz Abstract: We distinguish geometric cell structures using a deformable mirror for high-throughput, label-free flow cytometry, overcoming approaches that can result in undesirable chemical modifications or low throughput. Extended abstract Novel Deconvolution Kernel for Extended Depth-of-Field […]